DNA

Part:BBa_K1791000:Design

Designed by: Graeme Glaister   Group: iGEM15_Lethbridge   (2015-09-17)


Theophylline dependent ribozyme


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We have tested various temperatures, Magnesium and spermidine concentration under in vitro conditions but were unable to prevent the ribozmye from cleaving without the addition of theophylline. We will be investigating mutations to the theophylline ribozyme in order to optimize it for invitro conditions.


Source

Adapted from Win and Smolke (2007). A modular and extensible RNA-based gene-regulatory platform for engineering cellular function. PNAS. 104(36):14283-8.

References